Research Abstract

Collins, D, Parsa, B., Christensen, A., Schell, A., Miller, C., Nash, D., Bilsky, E. J., & Brosnan-Watters, G. (2000, November). Effects of MK-801 on cerebrocortical neuronal injury in six inbred strains of mice. Poster session presented at the meeting of the Society for Neuroscience, New Orleans, LA.

Antagonists of the NMDA glutamate (Glu) receptor, including Phencyclidine (PCP), ketamine, and MK-801 injure neurons in the posterior cingulate/retrosplenial (PC/RS) cortex in adult rats and mice and produce, in humans, a schizophrenia Ð like psychosis. We have previously reported that the neuronal injury, which consists of cytoplasmic vacuoles in layers III and IV of the PC/RS cortex, was not equally evident in three strains of mice, ICR, Non-Swiss Albino (NSA), and C57BL6/J (B6) mice. The two albino outbred strains had a mean of 40 to 50 damaged neurons per section, but the mean number of injured neurons for the B6 was 7. Here we report results in 6 other inbred strains, including SV129, AJ, Balb/C, DBA, Microphthalmic, and Meander. The histological examination consists of administering 1 mg/kg of MK-801 to the mice, sacrificing them via intracardiac perfusion 5 hours later, sectioning at 1 micron, and examining the PC/RS cortices using light microscopy. The number of injured neurons ranged from 0 to 41 per section in these strains. Although all strains of mice showed obvious behavioral reactions to the drug (including “popping” in some mice), differences in those reactions were also noted and quantified.